Determination of alkaline phosphatase (ALP) is used to assess the completeness of pasteurisation. Official reference method for the measurement of ALP is the fluorimetric method according to PN-EN ISO 11816. ALP activity is measured by fluorimetric estimation of kinetics of hydrolysis reaction of the substrate which, when acted upon ALP, is converted to a highly fluorescent product. The aim of this study was revalidation of the assay used for determination of ALP activity in cow milk, goat milk and cheese. The precision of the method was determined by assignation of repeatability and reproducibility. Moreover, the ALP activity in milk and cheeses from retail outlets was monitored. ALP activity in milk ranged from < 10 to 483 mU/l, most of samples had ALP activity below 250 mU/l. ALP activity in cheese ranged from < 0.5 to 90.46 mU/g, most of samples had ALP activity below 10 mU/g. Values of the precision of the method fitted the criteria established for interlaboratory comparisons which are included in standard
PN-EN ISO 11816.
Full text available in Polish in Adobe Acrobat format:https://www.npt.up-poznan.net/pub/art_3_132.pdf
| MLA | Rola, Jolanta Grażyna, and Maciej Sosnowski. "Zastosowanie metody fluorymetrycznej w ocenie skuteczności pasteryzacji mleka krowiego, koziego i serów." Nauka Przyr. Technol. 3.4 (2009): #132. |
| APA | Jolanta Grażyna Rola, Maciej Sosnowski (2009). Zastosowanie metody fluorymetrycznej w ocenie skuteczności pasteryzacji mleka krowiego, koziego i serów. Nauka Przyr. Technol. 3 (4), #132 |
| ISO 690 | ROLA, Jolanta Grażyna, SOSNOWSKI, Maciej. Zastosowanie metody fluorymetrycznej w ocenie skuteczności pasteryzacji mleka krowiego, koziego i serów. Nauka Przyr. Technol., 2009, 3.4: #132. |
Jolanta Grażyna Rola
Zakład Higieny Żywności Pochodzenia Zwierzęcego
Państwowy Instytut Weterynaryjny – Państwowy Instytut Badawczy w Puławach
al. Partyzantów 57
24-100 Puławy
Poland
e-mail: jolarola@piwet.pulawy.pl